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. 2016 Oct 12;157(12):4803–4816. doi: 10.1210/en.2016-1367

Figure 2.

Figure 2.

Expression of AMPKα2 in Kiss1 neurons and validation of the Kiss1-Cre AMPKα2loxP mouse model. A–D, Bright-field digital images showing the coexpression of AMPKα2 (silver grains) and Kiss1 (GFP immunoreactive [ir] cells) in the AVPV and Arc of male (A and B) and female (C and D) mice. E, PCR product showing DNA recombination in hypothalamus and liver of Kiss1-Cre AMPKα2loxP female mice (366 bp band, 100 bp DNA ladder). F, Graphs showing percentage of Kiss1 cells expressing AMPKα2 mRNA in control and Kiss1-Cre AMPKα2loxP female mice (n = 4/group). Unpaired two-tailed t test was used for the analysis. **, P < .05, ****, P < .0001 (t = 25.61, df = 4). F test to compare variance between groups showed no difference between groups for AVPV and Arc.