Table 1.
List of Antibodies Used in the Present Study
| Peptide/Protein Target | Antigen Sequence (if Known) | Name of Antibody | Manufacturer, Catalog Number, and/or Name of Individual Providing the Antibody | Species Raised in; Monoclonal or Polyclonal | Dilution Used | RRID |
|---|---|---|---|---|---|---|
| Ovine LHβ | oβLH | NIDDK | Rabbit; polyclonal | WB 1:50K; IHC 1:1K | AB 2629449 | |
| Ovine GnRH-R | FSQCVTHCS FPQWWHQA FYN | Ovine GnRH-R | Dr Donal Skinner (University of Wyoming) | Rabbit, polyclonal | WB 1:1K | AB 2629448 |
| AR | AR (N-20) | Santa Cruz Biotechnology, SC-816 | Rabbit; polyclonal | WB 1:1K | AB 1563391 | |
| ERα | ERα (clone 1D5) | Thermo Scientific, MS-354 | Mouse; monoclonal | WB 1:500 | AB 61341 | |
| ERα | ERα (clone 1D5) | Dako, M7047 | Mouse; monoclonal | IHC 1:100 | AB 2101946 | |
| IRβ | IRβ (Ab-6; clone CT-3) | Thermo Scientific, MS-636 | Mouse; monoclonal | WB 1:200 | AB 142261 | |
| PTEN | PTEN (D4.3) XP | Cell Signaling, 9188 | Rabbit; monoclonal | WB 1:1K | AB 2253290 | |
| GAPDH | GAPDH (14C10) | Cell Signaling, 3683 | Rabbit; monoclonal | WB 1:1K | AB 1642205 | |
| p-AKT | p-AKT (Thr308) (D25E6) | Cell Signaling, 13038 | Rabbit; monoclonal | WB 1:1K | AB 2629447 | |
| AKT | AKT 9pan) (C67E7) | Cell Signaling, 4691 | Rabbit; monoclonal | WB 1:1K | AB 915783 | |
| p-mTOR | p-mTOR (S2448) | Cell Signaling, 2971 | Rabbit; monoclonal | WB 1:1K | AB 330970 | |
| mTOR | mTOR | Cell Signaling, 2972 | Rabbit; monoclonal | WB 1:1K | AB 330978 | |
| p-ERK | p-p44/42 MAPK (T202/Y204) (D13.14.4E) | Cell Signaling, 4370 | Rabbit; monoclonal | WB 1:1K | AB 2315112 | |
| ERK | p44/42 MAPK (Erk1/2) (137F5) | Cell Signaling, 4695 | Rabbit; monoclonal | WB 1:1K | AB 390779 |
WB, Western blotting; IHC, immunohistochemistry.