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. 2016 Oct 25;157(12):4961–4972. doi: 10.1210/en.2016-1465

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Copyright © 2016 by the Endocrine Society

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Figure 2. — Exogenous T₃ induces dnmt3a mRNA and hnRNA in early prometamorphic X. tropicalis tadpole brain (middle brain region containing the preoptic area and diencephalon), tail, and hind limb. We treated early prometamorphic (NF stages 53–54) tadpoles with T₃ (5 nm) for 0, 2, 8, or 16 hours and measured dnmt3a mRNA and hnRNA by RTqPCR. We normalized dnmt3a mRNA and hnRNA to ef1a mRNA, which did not change with T₃ treatment (data not shown). Control reactions without reverse transcriptase were conducted on each RNA sample and produced no amplification. Bars represent the mean ± SEM (n = 4/time point/treatment), and means with the same letter (a, b, c for mRNA; A, B, C for hnRNA) are not significantly different (brain mRNA: F_(3,12) = 101.997, P < .0001; brain hnRNA: F_(3,12) = 76.795, P < .0001; tail mRNA: F_(3,12) = 67.891, P < .0001; tail hnRNA: F_(3,12) = 36.235, P < .0001; hind limb mRNA: F_(3,12) = 41.84, P < .0001; hind limb hnRNA: F_(3,12) = 32.83, P < .0001; ANOVA).