Figure 1.
Transglutaminase 2 (TG2) expression and in vivo activity in colon from dextran sulfate sodium (DSS)-treated mice. Wild-type (WT) and TG2−/− mice were treated with 3.5% DSS in drinking water for 5 days, and were intraperitoneally injected with biotin-amido-pentylamine (100 mg kg−1). After 3 h, mice were killed. Cryosections of colon were stained with anti-TG2 antibody, Texas Red-conjugated streptavidin and DAPI for TG2 protein, TG activity and nucleus detection, respectively. Data are representative of three experiments. CA, columnar absorptive cells; DAPI, 4',6-diamidino-2-phenylindole; DIC, differential interference contrast; G, goblet cells; LP, lamina propria; M, mucosa; MM, muscularis mucosae; NT, no treatment; SM, submucosa.