Figure 7.

Summary of changes in gene transcripts measured from whole flies after CrzR-RNAi with two different fat body Gal4 drivers. Knockdown of the CrzR in fat body does not induce a strong response in gene transcription at the level of whole flies during normal (fed) conditions. Data from qPCR are shown as average fold-changes of expression compared with level in the respective controls during normal conditions (see key for colour coding). Both experimental fly crosses, ppl>CrzR-Ri and to>CrzR-Ri, display similar patterns of gene transcript changes. Thus, TotA was upregulated during normal conditions in flies with CrzR-knockdown, but starvation results in reduced TotA. dilp6 mRNA is not affected in whole flies by any condition. Starvation leads to downregulation of Upd2, Sod2 and Nlaz, whereas bmm and pepck expression increases. In electronic supplementary material, figure S6, these experiments are shown in regular graphs.