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. 2016 Nov 3;6(11):160152. doi: 10.1098/rsob.160152

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© 2016 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 9. — Crz knockdown with a Crz-Gal4 driver generates phenotypes similar to peripheral receptor knockdown. (a) Knockdown efficiency of Crz-RNAi with the Crz-Gal4. Data are presented as means ± s.e.m., n = 4 independent replicates with 10 flies in each replicate (***p < 0.001, ANOVA followed with Tukey's test). (b) Resistance to desiccation stress is increased after Crz-RNAi. (c) Resistance to oxidative stress induced by paraquat feeding increases after Crz-RNAi (χ² = 54 and 137, p < 0.0001 comparing with the Gal4 and UAS control, respectively). (d) Food intake is reduced after Crz-RNAi. Accumulated food intake over 4 days of 3-day-old male flies (µl 96 h per fly) was estimated by CAFE assay (n = 4 independent replicates with eight flies in each replicate; ***p < 0.001, ANOVA followed with Tukey's test).