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. 2016 Nov 23;6(11):160162. doi: 10.1098/rsob.160162

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© 2016 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 1. — Evolution assay of Pseudomonas aeruginosa in H₂O₂. (a) Percentage of rough small colony variants (RSCVs) after evolution in the presence of H₂O₂ compared with the control. (b) Resistance of RSCV isolates compared with PAO1 in H₂O₂. (c) Competitive fitness assay between RSCV isolates and PAO1 grown in H₂O₂. (d) Antioxidants (glutathione; GSH) reduced the proportion of RSCVs formed with ROS treatment, in a dose-dependent manner. Means ± s.d. from triplicate experiments are shown.