Table 1.

Primer sequences and RT-PCR conditions. The annealing temperature was 60°C for all genes. F, forward primer; R, reverse primer.

gene	primer sequence	product size
Lin28a	F: 5'-GCGAAGATCCAAAGGAGACA-3'	206 bp
	R: 5'-TGTGGATCTCTTCCTCTTCC-3'
Oct4	F: 5'-CCGGAAGAGAAAGCGAACTA-3'	393 bp
	R: 5'-CAGTTTGAATGCATGGGAGA-3'
Klf4	F: 5'-AAAAGAACAGCCACCCACAC-3'	227 bp
	R: 5'-GAAAAGGCCCTGTCACACTT-3'
Sox2	F: 5'-AACCCCAAGATGCACAACTC-3'	201 bp
	R: 5'-TCCGGGAAGCGTGTACTTAT-3'
c-Myc	F: 5'-TGATGTGGTGTCTGTGGAGA-3'	230 bp
	R: 5'-TGTTGCTGATCTGCTTCAGG-3'
Nanog	F: 5'-CCAAAGGATGAAGTGCAAGC-3'	106 bp
	R: 5'-GCAATGGATGCTGGGATACT-3'
Dppa2	F: 5'-CACAGACTACGCTACGCAATCA-3'	245 bp
	R: 5'-AGTGTCTCCGAAGTCTCAAATAG-3'
Dppa4	F: 5'-GATACCTGCCCTCATTGACCCT-3'	182 bp
	R: 5'-CACACCACATTTCCCCTTTGACTTC-3'
Gata6	F: 5'-CAACCACTACCTTATGGCGTAGAAA-3'	354 bp
	R: 5'-GGCCGTCTTGACCTGAATACTTGA-3'
Piwil2	F: 5'-TTGTCATGTCGGACGGGAAGG-3'	320 bp
	R: 5'-CTCATTGCTGGCTGTCTCGTTTTGT-3'
Cbx1	F: 5'-CTACGAGCAGTGTCACCCTTCA-3'	295 bp
	R: 5'-TTGCCTCCCTCTGACTTATCTG-3'
Hdac3	F: 5'-TCCCGAGGAGAACTACAGCAGG-3'	280 bp
	R: 5'-GGACAATCATCAGGCCGTGAGA-3'
Tbpl1	F: 5'-CGGAACAACAAAGCGAGAAACC-3'	203 bp
	R: 5'-AGATCACATCCGTGGGAAGACG-3'
eIF-1a	F: 5'-TTTGGTCACTACTCAGGAGG-3'	149 bp
	R: 5'-ATCAGAAGCAACTGGGACAC-3'
Mt1a	F: 5'-CACCAGATCTCGGAATGGAC-3'	114 bp
	R: 5'-AGCAGCTCTTCTTGCAGGAG-3'
Cdc2	F: 5′-GGACTACAAGAACACCTTTC-3′	262 bp
	R: 5′-CAGGAAGAGAGCCAACGGTA-3′
Hsp70.1	F: 5′-AACGTGCTCATCTTCGACCT-3′	185 bp
	R: 5′-TGGCTGATGTCCTTCTTGTG-3′
Muerv-l	F: 5′-TTGCTTCCTGTCCCCATAAC-3′	132 bp
	R: 5′-AAAATGACCAGGGGGAAGTC-3′
Rpl23	F: 5′-CATGGTGATGGCCACAGTTA-3′	136 bp
	R: 5′-GACCCCTGCGTTATCTTCAA-3′
Ube2a	F: 5′-AATGGTTTGGAATGCGGTCA-3′	272 bp
	R: 5′-TGTTTGCTGGACTATTGGGA-3′
U2afbp-rs	F: 5′-TAAGCTGCAACCTGGAACCT-3′	109 bp
	R: 5′-CCTGCGTACCATCTTCCATT-3′
H1Foo	F: 5'-GCGAAACCGAAAGAGGTCAGAA-3'	377 bp
	R: 5'-TGGAGGAGGTCTTGGGAAGTAA-3'
Gapdh	F: 5'-ACCACAGTCCATGCCATCAC-3'	452 bp
	R: 5'-TCCACCACCCTGTTGCTGTA-3'