Figure 5.
The origin of the novel operon in the eustigmatophyte plastid genomes can be traced to a bacterium from the phylum Bacteroidetes. The phylogenetic tree displayed was inferred with the ML method and the GTR+Γ substitution model from a concatenated alignment of protein sequences of all six ebo genes (1838 aligned amino acids). Bootstrap support values are shown at branches when more than 75%. The tree is arbitrarily rooted on a maximally supported branch separating Cyanobacteria and other taxa included in the analysis. For simplicity, monophyletic groups comprising exclusively representatives of the same genus were collapsed and are shown as triangles, with the two side lengths proportional to the distances to closest and furthest leaves. Note that sequences from the eustigmatophytes Vischeria sp. CAUP Q 202 and Monodopsis sp. MarTras21 branch among members of the phylum Bacteroidetes, possibly specifically related to the ebo operon from Sporocytophaga myxococcoides. Note also that the cluster of sequences from the genus Leptospira (phylum Spirochaetes) is firmly nested among Bacteroidetes, indicating an inter-phylum HGT event.