Table 2.

Functional annotation of Ebo proteins.

protein	predicted function	details
EboA	novel protein, no functional assignment	no homologues identified even with HHsearch
EboB	putative hydrolase (but different enzymatic activities cannot be excluded)	assigned to the PF01026 family (‘TatD-related DNase’) by Pfam search, but appears to be a separate cluster of the same clan (CL0034 – ‘Amidohydrolase’; electronic supplementary material, figure S3); ‘Metallo-dependent hydrolase’ by SUPERFAMILY
EboC	putative prenyltransferase	UbiA superfamily of intramembrane prenyltransferases [50] based on blastp and Pfam searches (PF01040); proved to comprise a separate cluster within the superfamily (figure 6)
EboD	putative SPC, sedoheptulose 7-phosphate may be the substrate	belongs to SPC superfamily [66], equal to the PF01761 family (‘3-dehydroquinate synthase’) in Pfam, but a separate branch within the superfamily putatively with a novel specificity (figure 7) rather than true DHQS
EboE	TIM-barrel protein of the xylose isomerase-like superfamily, no more specific prediction of the enzyme activity possible	Some EboE sequences marginally similar to the PF01261 family (‘Xylose isomerase-like TIM barrel’) by Pfam search; ‘Xylose isomerase-like’ by SUPERFAMILY; homologous to TIM-barrel proteins according to HHsearch
EboF	putative hydrolase specific for a phosphate moiety-containing substrate	PF01663 family (‘Type I phosphodiesterase/nucleotide pyrophosphatase’) by Pfam search; ‘Alkaline phosphatase-like’ by SUPERFAMILY; a distinct phylogenetic lineage within the PF01663 family (electronic supplementary material, figure S2)