Table 1.
Strains and Plasmids Used in this Study
| Strain or Plasmid | Description | References |
|---|---|---|
| DSC0295 |
MATahis3Δ1 leu2Δ0 met 15Δ0 ura3Δ0; Tet-Off C-terminally TAP-tagged Ntg1 |
(24) |
| YSC1178-7499106 (DSC0297) |
MATahis3Δ1 leu2Δ0 met15Δ0 ura3Δ0; C- terminally TAP-tagged Ntg1 |
Open Biosystems |
| BY4147 (DSC0313) | MATahis3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | Open Biosystems |
| DSC0470 |
MATa
ntg1::hphMX4, his7-1, lys2Δ5'::LEU- lys2Δ3', ade5-1, trp1-289, ura3-52 |
This study |
| EJY341 (DSC0527) |
MATa
trp1-Δ1 ura3-52 his3-Δ200 leu2-3,112 lys2-801 [cir°] |
(99) |
| EJY342 (DSC0528) |
MATa
trp1-Δ1 ura3-52 his3-Δ200 leu2-3,112 lys2-801 siz1Δ::LEU2 [cir°] |
(99) |
| EJY343 (DSC0529) |
MATa
trp1-Δ1 ura3-52 his3-Δ200 leu2-3,112 lys2-801 siz2Δ::TRP1 [cir°] |
(99) |
| EJY344 (DSC0530) |
MATa
trp1-Δ1 ura3-52 his3-Δ200 leu2-3,112 lys2-801 siz1Δ::LEU2 siz2Δ::TRP1 [cir°] |
(99) |
| MHY1488 (DSC0534) | MATa ulp1Δ::HIS3 LEU2::ulp1-333 | (58) |
| EJY447 (DSC0535) |
MATa
trp1-Δ1 ura3-52 his3-Δ200 leu2-3,112 lys2-801 ulp2Δ::kanMX [cir°] |
(60) |
| GBY5 (DSC0536) | MATa smt3-allR::TRP1 | (55) |
| DSC0537 |
MATa
ntg1::hphMX4, his7-1, lys2Δ5'::LEU- lys2Δ3', ade5-1, trp1-289, ura3-52, pD0436 |
This study |
| DSC0538 |
MATa
trp1-Δ1 ura3-52 his3-Δ200 leu2-3,112 lys2-801 [cir°], pD0436 |
This study |
| DSC0539 | MATa smt3-allR::TRP1, pD0436 | This study |
| DSC0540 | MATa ulp1Δ::HIS3 LEU2::ulp1-333, pD0436 | This study |
| hDNP19 |
MATa/MATα rad1::kanMX/RAD1 ntg1::hphMX4/NTG1 ntg2::BSD/NTG2 apn1::TRP1/APN1 DSF1::URA3/DSF1 his7- 1/his7-1 lys2Δ5′::LEU-lys2Δ3′/lys2Δ5′::LEU- lys2Δ3′ ade5-1/ade5-1 trp1-289/trp1-289 ura3- 52/ura3-52 |
(19) |
| DSC0367 |
MATa his7-1 lys2Δ5′::LEU-lys2Δ3′ ade5-1 trp1- 289 ura3-52 |
(51) |
| DSC0369 |
MATa ntg1::hphMX4 rad1::kanMX ntg2::BSD apn1::TRP1 his7-1 lys2Δ5′::LEU-lys2Δ3′ ade5-1 trp1-289 ura3-52 |
(51) |
| DSC0371 |
MATa rad1::kanMX ntg2::BSD apn1::TRP1 his7-1 lys2Δ5′::LEU-lys2Δ3′ ade5-1 trp1-289 ura3-52 |
(51) |
| DSC0561 |
MATa ntg1k20,38,376,388,396R rad1::kanMX ntg2::BSD apn1::TRP1 DSF1::URA3 his7-1 lys2Δ5′::LEU-lys2Δ3′ ade5-1 trp1-289 ura3-52 |
This study |
| DSC0549 |
MATa ntg1k396R rad1::kanMX ntg2::BSD apn1::TRP1 DSF1::URA3 his7-1 lys2Δ5′::LEU- lys2Δ3′ ade5-1 trp1-289 ura3-52 |
This study |
| DSC0551 |
MATa ntg1k20,38R rad1::kanMX ntg2::BSD apn1::TRP1 DSF1::URA3 his7-1 lys2Δ5′::LEU- lys2Δ3′ ade5-1 trp1-289 ura3-52 |
This study |
| DSC0558 |
MATa ntg1k20,38,376,388R rad1::kanMX ntg2::BSD apn1::TRP1 DSF1::URA3 his7-1 lys2Δ5′::LEU- lys2Δ3′ ade5-1 trp1-289 ura3-52 |
This study |
| DSC0561 |
MATa ntg1k20,38,376,388,396R rad1::kanMX ntg2::BSD apn1::TRP1 DSF1::URA3 his7-1 lys2Δ5′::LEU-lys2Δ3′ ade5-1 trp1-289 ura3-52 |
This study |
| DSC0555 |
MATa ntg1k376,388,396R rad1::kanMX ntg2::BSD apn1::TRP1 DSF1::URA3 his7-1 lys2Δ5′::LEU- lys2Δ3′ ade5-1 trp1-289 ura3-52 |
This study |
| pD0390 | pET-15b His6-NTG1 | (51) |
| pD0394 | pET -15b His6-NTG1Δcat | (51) |
| pD0493 | pET -15b His6-NTG1(K->R)5 | This study |
| pD0436 | Tet-Off NTG1-TAP, CEN, URA3, ampR | This study |
| pD0437 | Tet-Off ntg1K20R-TAP, CEN, URA3, ampR | This study |
| pD0438 | Tet-Off ntg1K38R-TAP, CEN, URA3, ampR | This study |
| pD0444 | Tet-Off ntg1K376R-TAP, CEN, URA3, ampR | This study |
| pD0445 | Tet-Off ntg1K388R-TAP, CEN, URA3, ampR | This study |
| pD0446 | Tet-Off ntg1K396R-TAP, CEN, URA3, ampR | This study |
| pD0447 | Tet-Off ntg1K20,38R-TAP, CEN, URA3, ampR | This study |
| pD0448 | Tet-Off ntg1K20,38,376R-TAP, CEN, URA3, ampR | This study |
| pD0449 | Tet-Off ntg1K20,38,396R-TAP, CEN, URA3, ampR | This study |
| pD0450 | Tet-Off ntg1K20,38,376,388R-TAP, CEN, URA3, ampR |
This study |
| pD0451 | Tet-Off ntg1K20,38,376,388,396R-TAP, CEN, URA3, ampR |
This study |
| pD0452 | Tet-Off ntg1K376,388,396R-TAP, CEN, URA3, ampR | This study |