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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: DNA Repair (Amst). 2016 Oct 31;48:51–62. doi: 10.1016/j.dnarep.2016.10.011

Table 3.

Quantification of Mono-, Di-, Tri- Sumoylation of Ntg1 Variants

Potential
Sumo
Modification
Fraction of Modified Ntg1 (%)
WT K20R K38R K376R K388R K396R K20,
38R
K20,38,
376R
K20,38,
376,
388R
K20,38,
376,388,
396R
K376,
388,
396R
Ntg1-
Tri
0.31 0.11 0.02 0.18 0.13 0.00 0.36 0.02 0.00 0.00 0.00
Ntg1-
Di
0.49 0.14 0.00 0.23 0.21 0.18 0.00 0.05 0.00 0.00 0.00
Ntg1-
Mono
6.52 5.53 4.27 5.30 4.98 3.45 5.50 4.66 4.51 0.04 1.24