Table 3.
Potential Sumo Modification |
Fraction of Modified Ntg1 (%) | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | K20R | K38R | K376R | K388R | K396R | K20, 38R |
K20,38, 376R |
K20,38, 376, 388R |
K20,38, 376,388, 396R |
K376, 388, 396R |
|
Ntg1- Tri |
0.31 | 0.11 | 0.02 | 0.18 | 0.13 | 0.00 | 0.36 | 0.02 | 0.00 | 0.00 | 0.00 |
Ntg1- Di |
0.49 | 0.14 | 0.00 | 0.23 | 0.21 | 0.18 | 0.00 | 0.05 | 0.00 | 0.00 | 0.00 |
Ntg1- Mono |
6.52 | 5.53 | 4.27 | 5.30 | 4.98 | 3.45 | 5.50 | 4.66 | 4.51 | 0.04 | 1.24 |