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. 2016 Dec 2;6:38221. doi: 10.1038/srep38221

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Copyright © 2016, The Author(s)

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(A) The percentage of ALDH-positive esophageal cancer cells increase under laminar flow in the microchannel. FACS analysis of ALDH expression of cancer cells under static and flow conditions on day 7. (B) ALDH-positivity percentages of esophageal cancer cells under static and flow conditions on day 1, 3, 5 and 7. (C) Microscopic images of colony forming assay under static and flow conditions. (D) Bar graphs demonstrate the colony number under static and flow conditions. The colony forming capacity of esophageal cancer cells increases under laminar microfluidic flow (*p ≤ 0.05). (E, F, G) FACS analysis of CD24 and CD44 expression at different time points of esophageal cancer cells under static and laminar flow conditions (*p ≤ 0.05). All data were expressed as mean ± S.E.M., n = 3, star (*) indicates, p < 0.05.