Figure 2. The fabrication and design of our paper-based CRP monitoring devices.

(a) Fabrication of the paper-based diagnostic device via wax printing. (b) An optical image of the paper-based diagnostic device with one channel for whole blood testing. (c) Schematic drawing of our paper-based diagnostic device designed for CRP assays (negative channel, sample channel, positive channel – a gradient for reagent concentration). Latex was loaded from the 3 outer circles for immobilization. Blood sample was loaded into the center circle for testing. (d) Actual picture of experiment result shows the profiles of one CRP assay on our paper-based diagnostic device (paper chromatography).