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. 2016 Dec 2;6:38372. doi: 10.1038/srep38372

Figure 1. Characterization of the 55 kDa protein derived from the plasma membrane proteins of ECV304 cells.

Figure 1

(A) Western blot analysis of the 55 kDa protein. The total ECV304 cell proteins (lane 1), ECV304 cell plasma membrane proteins (lane 2), and the 55 kDa proteins (lane 3) excised from an SDS-PAGE gel of the plasma membrane proteins of ECV304 cells were separated by 12% SDS-PAGE and analyzed with commercial rabbit anti-vimentin PcAb as described in Materials and Methods. Protein sizes are indicated on the left. (B) Co-immunoprecipitation (Co-IP) experiment. The mixture of the ECV304 cell plasma membrane proteins and the purified DENV-2 EDIII was either directly analyzed (lane 1, served as protein controls) or analyzed after Co-IP assay with commercial rabbit anti-vimentin PcAb (upper panel) or mouse anti-DENV-2 EDIII PcAb (bottom panel) as indicated by an arrow on the right for testing the presence of proteins of interest (lane 3). Co-IP without DENV-2 EDIII proteins (lane 2) or with an isotype Ab (anti-TNFα, lane 4) served as controls. The full-length blots are presented in Supplementary Figure 1.