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. 2016 Dec 2;6:38372. doi: 10.1038/srep38372

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) Flow cytometric analysis of superficial vimentin on DENV2-infected ECV304 cells at 15, 38, and 62 h post-infection (hpi), and 0 h infected cells were used as control. (B) Quantitative analysis of superficial vimentin on DENV2-infected ECV304 cells at the indicated time points. Data are presented as means ± standard deviation (S.D.) (n = 3). P-values were indicated versus 0 h infection. (C) IFA of DENV-2 infected ECV304 cells at the indicated time points. The DENV-2 infected ECV304 cells were detected with the mouse anti-DENV-2 EDIII pcAb, and cell nuclei were stained by DAPI as described in Materials and Methods. After washing, the cells were observed under a confocal laser microscope (magnification, ×400).