Figure 5. Sensitivity of different T/F viruses to IFN-α inhibition.

The replication kinetics of each multiple T/F virus in the absence (solid lines) and presence of 500 U/ml IFN-α (dashed lines) from subjects CH0200 (A), CH0228 (B), CH0078 (C) were determined in primary CD4 T cells from a health donor C24. Activated primary CD4 + T cells from donor C24 were infected with equal amount (500 TCID₅₀) of each virus. Viral replication was monitored by measuring the p24 concentration in the culture supernatant daily for 7 days. All infections were performed in triplicates. The resistance to IFN-α inhibition by different T/F viruses from CH0200 (D), CH0228 (E), CH0078 (F) was determined by comparing the ratio of viral p24 production in the presence and absence of IFN-α at day 7. The resistance to IFN-α inhibition by different T/F viruses from CH0200 (G), CH0228 (H) and CH0078 (I) was also determined in primary CD4 + T cells from another donor C29 at day 7.