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. 2016 Dec 2;6:38491. doi: 10.1038/srep38491

Figure 2. PPARγ and GREM1 were direct targets of miRNA-27a.

Figure 2

(a) PPARγ and GREM1 share a matching 3′UTR sequence that targets the seed region of miR-27a. The sequences are presented. (b) Western blots showed the down-regulation of PPARγ and GREM1 in the miR-27a mimics group and up-regulation of PPARγ and GREM1 in the miR-27a inhibitor group compared with the mimics or inhibitor control groups. (c, d) The dual luciferase assay showed significantly reduced luciferase activity in BMSCs that were co-transfected with the miR-27a mimics and pmirGLO-wt-PPARγ compared with cells co-transfected with miR-27a mimics and pmirGLO-mt-PPARγ, and significantly enhanced luciferase activity was observed in BMSCs that were co-transfected with miR-27a inhibitor and pmirGLO-wt-PPARγ compared with the cells that were co-transfected with the miR-27a inhibitor and pmirGLO-mt-PPARγ. A similar result was observed in BMSCs that were co-transfected with the miR-27a mimics and pmirGLO-wt-GREM1 or the cells that were co-transfected with the miR-27a inhibitor and pmirGLO-wt-GREM1. *P < 0.05.