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. 2016 Dec 2;6:38491. doi: 10.1038/srep38491

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Copyright © 2016, The Author(s)

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(a) Oil red O staining detected more lipid droplets in BMSCs from the Model group compared with the Control group on day 14. (b) A significant increase in the TG content of BMSCs from the Model group was observed in comparison with the Control group on days 7 and 14. (c,d) Significant decreases in the ALP and OST contents in the Model group were observed in comparison with the Control group. (e,f,i) qRT-PCR showed that PPARγ, GREM1, and C/EBPα were significantly up-regulated in BMSCs from the Model group compared with the Control group. (g,h,j) qRT-PCR showed that Runx2, Bmp-2 and miR-27a were significantly down-regulated in BMSCs from the Model group compared with the Control group. (k) The western blotting results were consistent with the qRT-PCR results: PPARγ, GREM1, and C/EBPα were significantly up-regulated while Runx2 and Bmp-2 were significantly down-regulated in BMSCs from the Model group. *P < 0.05.