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. 2016 Dec 2;6:38199. doi: 10.1038/srep38199

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Copyright © 2016, The Author(s)

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(A) Immunohistochemistry was performed on sections of OE isolated from Ascl3^EGFP-Cre/+/R26^tdTomato/+ mice (2 months) using antibodies to EGFP and RFP. (B–D) Immunohistochemistry was performed on sections of OE isolated from Ascl3^EGFP-Cre/+ mice (2 months), using antibodies to EGFP and (B) NPY, (C) TuJ1, (D) CK5. Arrowheads indicate co-localization of EGFP and NPY. (E) In situ hybridization for Ascl3 mRNA in the OE of 2-month old mice (arrowheads). Dotted line indicates basal lamina. Nuclei are stained by DAPI (blue). Scale bars: (A–D), 25 μm. (E), 20 μm.