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. 2016 Dec 2;6:38308. doi: 10.1038/srep38308

Figure 4. MSCs modulate human primary macrophage differentiation and activation states.

Figure 4

(a,b) Fold increase in prototypic mRNA transcripts associated with inflammatory macrophages, M1 (a) and alternatively activated macrophages, M2 (b) upon co-cultures of naïve macrophages (Mo), M1 polarized (M1) or M2 polarized macrophages (M2) with MSCs. RQ was obtained after normalizing with mRNA levels in naïve primary macrophages (n = 3, Mean ± SEM). (c) Cytokine levels of TNFα, IL6 and IL10 in supernatants of naïve macrophages (Mo), M1 and M2 macrophages with (white bars) or without (black bars) MSC co-culture (n = 3). (d,e,f) Representative flow cytometry assessment of CD80/CD86, CD36/CD163 and CX3CR1/CD206 expressing cell populations in naïve Mo (d), M1 polarized (e) and M2 polarized macrophages (f) with (+MSC) or without MSC (−MSC) co-culture. Values in each quadrant of the dot plots depict mean % cells in each population (either double positive, single positive or double negative for the combination of cell surface antigens analyzed).