Figure 3. Effect of the C allele of rs35767 on IGF1 promoter activity.

(a) There was no significant difference in promoter activity between haplotype T-T-T and SacI digested haplotype C-T-T. (b) Compared to the promoter activity of haplotype C-T-T, which has been described previously²⁹, a significant decrease in promoter activity was observed in plasmids with low microsatellite repeat numbers, 17 or 18 repeats. Relative luciferase activity is shown as mean ± SD. One-way ANOVA was used for group comparison among four STRs (in a) and student’s t test was used for comparison of two haplotypes (in b). Each assay was repeated for four times in each of the four independent experiments (n = 16) (*p < 0.05; **p < 0.01 by student’s t test).