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. 2016 Dec 2;6:38281. doi: 10.1038/srep38281

Figure 3. Chromosome expansion induced by calcium.

Figure 3

(a) Effect of calcium on chromosome area. A histogram represents chromosome area of PA chromosomes treated with 0, 1, 3, and 7 mM CaCl2 in XB buffer (n = 100 chromosomes). (b) Average chromosome area in chromosome spreads after depletion (BAPTA-AM) and re-addition (CaCl2) of calcium (n = 7 chromosome spreads from three experiments). Error bars indicate standard deviations. Bars: (a) 2 μm and (b) 10 μm.