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. 2016 Dec 2;6:38228. doi: 10.1038/srep38228

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) Strategy for the construction of miRNA-overexpressing vector, which includes PsaD promoter (PPsaD), luciferase gene, and Cre-miR906-3p/Cre-miR910 miRNA-overexpressing precursor; (B) Screening Cre-miR906-3p-overexpressing cell lines by determining luciferase activity; (C) Screening Cre-miR910-overexpressing cell lines by determining luciferase activity; (D) q-PCR validation of the expression level of Cre-miR906-3p in the cell lines; (E) q-PCR validation of the expression level of Cre-miR910 in the cell lines. The expression folds of miRNA and its target gene were measured in the same samples; *Indicates that differences between the overexpressing lines and the wild-type lines were significant at P < 0.05.