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. 2016 Dec 2;6:38204. doi: 10.1038/srep38204

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Copyright © 2016, The Author(s)

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(a,b) p922 promoter constructs were transformed into wild-type, ∆hha, ∆tomB and ∆hha:tomB; and the expression of GFP was analysed at indicated time points after growth in LB (a) or minimal media (b). Reduced GFP expression was observed in ∆hha and ∆tomB in both media. (c) Level of Hha mRNA transcript in wild-type and ΔtomB. Overnight grown cultures of wild-type and ΔtomB were subcultured and mRNA was isolated with trizol method. ΔtomB showed increased mRNA transcript as compared to wild type. (d) Electrophoretic mobility shift assay was performed by incubating 100 ng of p922 PCR amplicon with increasing concentration of TomB from 10 μM to 100 μM. A shift in DNA band was observed with increasing concentration of TomB confirming that it binds to p922 promoter.