Skip to main content
. 2016 Nov 23;11:126–134. doi: 10.1016/j.redox.2016.11.009

Fig. 1.

Fig. 1.

Effects of high glucose concentrations on Rac1, NADPH oxidase activity and apoptosis. (a) INS-1 cells were treated with high concentrations of glucose (30 mM) for varying periods of time. Rac1 activity was measured by Rac1-Upstate assay kit. (*P<0.005 vs. Control). (b) INS-1 cells were treated with high concentrations of glucose (30 mM) for the indicated time periods. NADPH oxidase activity was measured by lucigenin based assay. Data represent the mean±SEM of three independent experiments. *P<0.001 vs Control. (c) INS-1 cells were treated with high concentrations of glucose (30 mM) for 24 h and apoptosis was assessed by TUNEL-assay using fluorescence microscopy. The results are expressed as the mean±SEM. *P<0.001 vs. Control.