Fig. 3.

Effects of NSC23766 on high glucose-induced CD36 expression. (a) Cells were treated with NSC23766 (50 μM) for 2 h and then exposed to high concentrations of glucose (30 mM) for the indicated periods of time. Total cell lysates was used to measure CD36 expression by immunoblotting. ^*P<0.001 vs. control, ^**P<0.005 vs. HG. (b, c) The effect of NSC237669 (50 μM) or Rac1-siRNA on high glucose induced CD36 expression at plasma membrane fractionation was analyzed by immunoblotting. Cellular plasma membrane were prepared using a plasma membrane protein extraction kit. NA⁺K⁺ATPASE was used as the loading control. ^*P<0.0001 vs. control, ^*P<0.001 vs. HG.