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. 2016 Nov 23;11:126–134. doi: 10.1016/j.redox.2016.11.009

Fig. 5.

Fig. 5.

Inhibitory effects of NSC23766 on high glucose-induced Rac1, NADPH oxidase and CD36 activation in human 1.1b4 cells. (a, b) Human pancreatic 1.1b4 cells were incubated with NSC23766 (50 μM) for 2 h and then exposed to high glucose concentrations (30 mM) for 24 h. Rac1 (*P<0.05 vs. control, **P<0.05 vs. HG) and NADPH oxidase (*P<0.001 vs. control, **P<0.001 vs. HG) activity were measured as described in the methods section. The results represent the mean±SEM of three independent experiments. (c) Human pancreatic 1.1b4 cells were treated with NSC23766 (50 μM) for 2 h and then exposed to high glucose concentrations (30 mM) for 24 h. Cellular plasma membrane extracts were prepared using a plasma membrane protein extraction kit. Plasma membrane fractionation cell lysates were analyzed by western blotting with CD36 or NA+K+ATPASE antibodies. *P<0.001 vs. control, **P<0.001 vs. HG. Data represents three independent experiments.