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. 2016 Nov 25;7:13597. doi: 10.1038/ncomms13597

Figure 1. miR-103 is upregulated during DNA damage and exacerbates DNA ds breaks.

Figure 1

(a) Venn diagrams showing miR profiling results from HUVECs treated with the indicated genotoxic agents and (b) expression levels of the top seven upregulated miRs (at least 1.5-fold cutoff). The numbers indicate fold change over control treated HUVECs normalized to a housekeeping small RNA at 6 h post treatment. (c) Heatmap depicting expression of miR-103 in different vascular cell types 6 h after radiation. Numbers depict mean fold increases over mock-treated cells. (d) qRT-PCR showing the expression of miR-103 in ECs 3 h post radiation in a 4T1 mammary carcinoma model. N=3 mice per group and two tumours per mouse. Bars show mean+s.e.m. * indicates P<0.05 by two-tailed Student's t-test. (e) qRT-PCR assay showing induction of miR-103 transcript (pri-miR) in HUVECs over time after a single 20 Gy dose of radiation. Mean+s.e.m. of two biological replicates is shown (f) qRT-PCR of pri-miR-103 in HUVECs 1 h after indicated doses of radiation. One of two representative experiments (g,h) Phosphorylation of histone H2AX in HUVECs measured by immunofluorescence staining 24 h after ectopic expression of miR-103 (g) and inhibition of miR-103 (h) 3 h after exposure to the indicated doses of radiation. Bars show mean+s.e.m. of pixel area normalized to the number of nuclei for 25–50 nuclei per group. * indicates P<0.05 by two-tailed Student's t-test. qRT-PCR; quantitative real-time PCR.