Figure 6. NPM1 dissociates from rRNA/rDNA after exposed to oxidized GSH in vitro.

SPR analysis of the interaction of NPM1-C70 (WT or C275S, as analyte) and G-quadruplexes (rDNA (upper) or rRNA (bottom), as ligand). Different colourful traces referred to increasing analyte concentrations (μg ml⁻¹). The dissociation constant (K_D) values were calculated with a one-site steady state binding model (see Supplementary Fig. 5g and Table 1). GSSG, oxidized GSH. G4, G-quadruplex.