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. 2016 Oct 27;17(11):1124. doi: 10.3390/ijms17111124

Figure 3.

Figure 3

Prediction and identification of the miR-25 target. (A) Diagram for construction of the miR-25 binding site in the pGL3 vector. Mutation analysis of the miR-25 binding site; (B) identification of the miR-25 target gene. HEK293 cells were co-transfected with miR-NC, miR-25, and DKK3-pGL3 for the dual-luciferase assay. Renilla luciferase was expressed in pRL-TK vector, and was co-transfected with 3′-UTR of DKK3 for normalization; (C,D) DKK3 protein levels in miR-25 or anti-miR-25-treated HEK293 cells were measured by Western blot analysis. GAPDH used as an internal standard. * p < 0.05.