Figure 4.

Overexpression of DKK3 attenuates the oncogenic influence of miR-25 in part. (A) MV3 were transfected with pcDNA3.1-DKK3 or control vector, and the protein levels of DKK3 were detected by Western blot analysis; (B) MV3 were co-transfected with miR-25 and pcDNA3.1-DKK3 vector or control, and the proliferation of MV3 was tested by the MTT assay; (C,D) MV3 motility of pretreated cells were measured by wound-healing assays; (E,F) pretreated MV3 motility was measured by transwell assays. Data are expressed as the mean ± SD of three independent experiments. * p < 0.05. Scale bar = 100 μm.