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. 2016 Oct 25;17(11):1777. doi: 10.3390/ijms17111777

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Changes of endogenous melatonin and polyamine content in response to Fe deficiency, and the effects of exogenous melatonin on cellular polyamine levels in Arabidopsis plants. Seven-day-old seedlings were placed in 1/2 MS liquid medium with or without the presence of 50 μM Fe for the indicated time, then these plants were taken to measure melatonin content (A). In addition, seve-day-old seedlings were treated with or without 5 μM melatonin under −Fe and +Fe conditions for 8 days. These plants were then used to determine cellular polyamine, including Put (B), Spd (C), and Spm (D). Each bar is the mean ± SE of at least three replicates, and different lowercase letters above the bars indicating significant differences using a Tukey’s test at p < 0.05.