Figure 2.

The effect of exogenous melatonin on Fe deficiency symptoms in the WT, adc2-1, and d-arg-treated Arabidopsis plants. Seven-day-old seedlings were grown under +Fe (50 μM Fe) and −Fe (0 μM Fe) conditions for 8 days, then these plants were sampled to measure the content of endogenous polyamine, including Put (A), Spd (B), and Spm (C). Additionally, seven-day-old seedlings were grown under +Fe and −Fe conditions with or without the presence of 5 μM melatonin for 8 days, respectively. Then, these plants were used to analyze the growth phenotype (D) and chlorophyll content (E). Each bar is the mean ± SE of at least three replicates, and different lowercase letters above the bars indicate significant differences using Tukey’s test at p < 0.05.