Figure 4.

The effect of exogenous melatonin on Fe content in the WT, adc2-1 and d-arg-treated Arabidopsis plants. Seven-day-old seedlings were treated with or without 5 μM melatonin in the presence or absence of 50 μM Fe for 8 days. Then these plants were taken to measure the soluble Fe content of shoots (A) and roots (B), the total Fe content of shoots (C) and roots (D), and to measure the Fe content in root cell wall components: total cell wall (E), pectin (F), HC1 (G), and HC2 (H) fractions. Each bar is the mean ± SE of at least three replicates, and different lowercase letters above the bars indicate significant difference using Tukey’s test at p < 0.05.