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. 2016 Oct 25;17(11):1777. doi: 10.3390/ijms17111777

Figure 10.

Figure 10

The effect of exogenous melatonin on the FCR activities and the expression of Fe-acquisition-related genes in polyamine- (adc2-1 and d-arg-treated) and NO-deficient (nia1nia2noa1 and c-PTIO-treated) Arabidopsis plants. Seven-day-old seedlings were treated with or without 5 μM melatonin in the presence or absence of 50 μM Fe for 8 days. Roots from adc2-1 and d-arg-treated plants were sampled for measuring the FCR activities (A). The expression levels of Fe acquisition-related genes, including FIT1 (B), FRO2 (C), and IRT1 (D), were examined after seven-day-old seedlings were placed in the −Fe or +Fe medium with or without the presence of 5 μM melatonin (−MT or +MT) for 8 days. In addition, roots from nia1nia2noa1 and c-PTIO-treated plants were used to determine the FCR activities (E). Each bar is the mean ± SE of at least three replicates, and different lowercase letters above the bars indicate significant differences using Tukey’s test at p < 0.05.