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. 2016 Oct 27;17(11):1798. doi: 10.3390/ijms17111798

Figure 3.

Figure 3

Effects of ganodermanondiol (GN) on cellular melanogenesis-related protein and microphthalmia-associated transcription factor (MITF) protein expression in B16F10 cells, pretreated with various concentrations of GN (2.5, 5, 7.5 and 10 μM) for 24 h following treatment with or without α-melanocyte-stimulating hormone (α-MSH) for 24 h. (A) Cellular proteins levels were examined using Western blot analysis; (B) Relative protein expression levels of tyrosinase, tyrosinase related protein 1 (TRP-1), TRP-2, and MITF protein. Relative protein expression levels were calculated using TL-100 software program (TotalLab, Newcastle, UK). Values are means ± standard deviation (SD) of three independent experiments. Statistical significance of differences was evaluated using a one-way analysis of variance (ANOVA) followed by Tukey’s test. *** p < 0.001 versus B16F10 cells without GN treatment.