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. 2016 Oct 29;17(11):1811. doi: 10.3390/ijms17111811

Figure 2.

Figure 2

Deletion and reconstitution of afpab1 of A. fumigatus. (a) The constructed schematic of Δafpab1 and afpab1C in A. fumigatus; (b) Molecular analysis of WT, Δafpab1 and afpab1C. Hph gene can be amplified from Δafpab1 (I) but cannot be amplified from WT or afpab1C, and afpba1 gene can be amplified from WT and afpab1C (II) which cannot be amplified from Δafpab1. Genomic DNA digested with Pst I was probed with an upstream region of afpab1 to detect the Δafpab1, WT, and afpab1C (III); (c) Southern hybridization schematic diagram. M: DNA molecular size marker Trans 2K plus II; 1: WT; 2: Δafpab1; 3: afpab1C. (Green: upstream fragment of afpab1 gene, Red: hph gene, Blue: downstream fragment of afpab1 gene).