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. 2016 Oct 29;17(11):1811. doi: 10.3390/ijms17111811

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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DCFH-DA staining was used to determine the ROS levels in the protoplasts treated with 3 mM H₂O₂ for 1 h. The conidia were observed using fluorescence microscopy to determine the ROS levels; Control group without H₂O₂. Bar, 10 µm.