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. 2016 Nov 1;17(11):1824. doi: 10.3390/ijms17111824

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Respiratory chain (RC) complex assembly and respiration in liver mitochondria. (A) BNGE analyses show respiratory chain complexes in liver mitochondria as follows: NDUFV1 (CI), NADH dehydrogenase (ubiquinone) complex I; CII, Complex II; CIII, Complex III; CIV, Complex IV; PDHE1α, pyruvate dehydrogenase E1; and ETFAα, electron transport flavoprotein alpha-polypeptide. HCD had no effect on RC complex assembly; (B) Liver mitochondrial respiration at coupled and uncoupled state; uncoupling between rate of respiration and ATP production was induced by carbonilcyanide p-tri-flouro-methoxy-phenyl-hydrazone (FCCP) (n = 3–4). HCD had no effect on respiration. SC1, supercomplex. p-Values: * p < 0.05.