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. 2016 Nov 5;17(11):1845. doi: 10.3390/ijms17111845

Figure 1.

Figure 1

Real-time reverse-transcription PCR (RT-PCR) analysis of marker gene expression in non-small cell lung carcinoma (NSCLC) tissues and paired peripheral blood mononuclear cell (PBMC) specimens. Total amount of mRNA was normalized to GAPDH and the relative mRNA expressions of the samples (tumor vs. para-cancerous histological normal tissues (PCHNTs), or paired PBMCs vs. positive control) were calculated using 2−ΔΔCt formula. (A) Tumor tissues vs. PCHNTs from 111 NSCLC patients. The mRNA expression of CK7, ELF3, EGFR and EphB4 in lung cancer tissues were significantly higher than that in PCHNTs (all p < 0.0001); T: Tumor tissues; P: PCHNTs; (B) PBMCs from 111 NSCLC patients vs. positive reference value (PRV). The mRNA expression of CK7, ELF3, EGFR and EphB4 in PBMCs from NSCLC patients were significantly higher than that in healthy controls, respectively (all p < 0.0001); P: PBMC; R: PRV. *: p ˂ 0.0001.