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. 2016 Nov 9;17(11):1868. doi: 10.3390/ijms17111868

Figure 2.

Figure 2

(A) Schema representing the formation of neutral lipid droplets from saturated free fatty acids (FAs) such as palmitate and stearate; (B) Representative immunoblot of cleaved caspase 3 and SCD1 in cultured proximal tubular cells infected with the control retrovirus (pBABE-CONT) or the retrovirus for SCD1 overexpression (pBABE-SCD1), and treated with/without palmitate or oleate. β-actin was used as a loading control; (C) Quantitative result of cleaved caspase 3 (n = 5); (D) Representative pictures of TUNEL staining under the indicated conditions. White arrows indicate TUNEL-positive apoptotic cells; (E) Quantitative results of TUNEL staining; (F) Representative immunoblot of cleaved caspase 3 and SCD1 in cultured proximal tubular cells transfected with the control siRNA or siRNA against SCD1, and treated with/without palmitate or oleate; (G) Quantitative result of cleaved caspase 3 (n = 7). SCD, stearoyl-CoA desaturase. Data are shown as mean ± SD. p < 0.05 indicates statistical significance.