Figure 3.
(A) Representative immunoblot of pPERK, PERK, sXBP1 and CHOP in cultured proximal tubular cells infected with the control retrovirus (pBABE-CONT) or the retrovirus for SCD1 overexpression (pBABE-SCD1), and treated with/without palmitate or oleate. β-actin was used as a loading control. Quantitative results of the pPERK/PERK ratio (B), sXBP1 (C) and CHOP (D) (n = 4). Data are shown as mean ± SD. p < 0.05 indicates statistical significance. pPERK, PKR-like ER kinase (PERK) phosphorylated at Thr980; sXBP1, spliced X-box binding protein 1; CHOP, C/EBP homologous protein; SCD, stearoyl-CoA desaturase.