Figure 3.

Short-chain fatty acid (SCFA) identification by NMR analysis. The media of S. epidermidis ¹³C₁₂-sucrose fermentation were centrifuged and passed through a 0.2 μm filter. Supernatants were then mixed with 10% D₂O and analyzed by NMR spectrometers. A 2-D ¹H-¹³C HSQC NMR spectrum (600 MHz) was displayed. The un-metabolized ¹³C₁₂-sucrose (solid arrows) appears 2.5–6.0 and 50–100 ppm in the ¹H- and ¹³C-NMR spectra, respectively. Besides sucrose, four SCFAs (acetic (Ac), butyric (BA), lactic (LA), and succinic acids (SA), open arrows) were detected in the ferments of S. epidermidis.