Characterization of culture pellets and supernatants of V. parahaemolyticus PCU-1 treated with MIC of NaOH. (A) Total proteins extracted from culture pellets of untreated bacterial cells (lanes 1–4: 15, 30, 45, and 60 min) and NaOH-treated VPGs (lanes 5–8: 15, 30, 45, and 60 min). M: Protein Marker; (B) Total proteins extracted from culture supernatants of untreated (lane 1) and NaOH-treated bacterial cells for 60 min (lane 2). Agarose gel (1%) electrophoresis of culture pellets containing VPGs (C); and culture supernatants (D). M: 1 kb Marker Ladder, lane 1: untreated V. parahaemolyticus PCU-1 (60 min) and lane 2: NaOH-treated V. parahaemolyticus PCU-1 (60 min).