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. 2016 Nov 16;17(11):1909. doi: 10.3390/ijms17111909

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) RT-qPCR quantification of the β-catenin expression level (n = 6). Red bars represent the negative control; black bars represent miR-30e-5p mimic or inhibitor; (B) The effect of miR-30e-5p mimicking and inhibiting β-catenin protein expression was evaluated by Western blot analysis in GMEC; (C) GMEC were transfected with miR-30e-5p mimic or inhibitor, and the miR-15a expression level was quantified by RT-qPCR (n = 6). Red bars represent the negative control; black bars represent miR-30e-5p mimic or inhibitor; (D) GMEC were transfected with miR-15a mimic or inhibitor, and the miR-30e-5p expression level was quantified by RT-qPCR (n = 6). Red bars represent the negative control; black bars represent miR-15a mimic or inhibitor; (E) the YAP1 expression level was quantified by RT-qPCR (n = 6). Red bars represent the negative control; black bars represent the miR-30e-5p mimic or inhibitor; (F) The effect of miR-30e-5p mimicking and inhibiting YAP1 protein expression was evaluated by Western blot in GMEC; (G) GMEC were transfected with Si-NC (60 nM) or SiRNA-β-catenin (60 nM) for 48 h, the mRNA expression of YAP1 was quantified by RT-qPCR (n = 6); (H) The effect of Si-NC (60 nM) or SiRNA-β-catenin (60 nM) for 48 h on YAP1 protein expression was evaluated by Western blot in GMEC. All experiments were duplicated and repeated three times. Values are presented as means ± standard errors, * p < 0.05; ** p < 0.01.

(A) RT-qPCR quantification of the β-catenin expression level (n = 6). Red bars represent the negative control; black bars represent miR-30e-5p mimic or inhibitor; (B) The effect of miR-30e-5p mimicking and inhibiting β-catenin protein expression was evaluated by Western blot analysis in GMEC; (C) GMEC were transfected with miR-30e-5p mimic or inhibitor, and the miR-15a expression level was quantified by RT-qPCR (n = 6). Red bars represent the negative control; black bars represent miR-30e-5p mimic or inhibitor; (D) GMEC were transfected with miR-15a mimic or inhibitor, and the miR-30e-5p expression level was quantified by RT-qPCR (n = 6). Red bars represent the negative control; black bars represent miR-15a mimic or inhibitor; (E) the YAP1 expression level was quantified by RT-qPCR (n = 6). Red bars represent the negative control; black bars represent the miR-30e-5p mimic or inhibitor; (F) The effect of miR-30e-5p mimicking and inhibiting YAP1 protein expression was evaluated by Western blot in GMEC; (G) GMEC were transfected with Si-NC (60 nM) or SiRNA-β-catenin (60 nM) for 48 h, the mRNA expression of YAP1 was quantified by RT-qPCR (n = 6); (H) The effect of Si-NC (60 nM) or SiRNA-β-catenin (60 nM) for 48 h on YAP1 protein expression was evaluated by Western blot in GMEC. All experiments were duplicated and repeated three times. Values are presented as means ± standard errors, * p < 0.05; ** p < 0.01.