Figure 9.

(A) Triglyceride levels in transfected cells were compared with that of control (n = 6). Red bars represent the negative control; black bars represent miR-15a mimic or inhibitor; (B) Cholesterol levels in transfected cells were compared with that of the control (n = 6). Red bars represent the negative control; black bars represent siRNA-YAP1; (C) The expression of β-casein was quantified by RT-qPCR (n = 6); (D) The effect of si-NC (60 nM) or siRNA-YAP1 (60 nM) on β-casein protein expression was evaluated by Western blot in GMEC; (E) Triglyceride levels in cells transfected with control inhibitor (50 nM) + control siRNA (50 nM), inhibitor 15a (50 nM) + Control siRNA (50 nM), and inhibitor 15a (50 nM) + siRNA-YAP1 (50 nM). All experiments were duplicated and repeated three times. Values are presented as means ± standard errors, * p < 0.05; ** p < 0.01.