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. 2016 Nov 16;17(11):1912. doi: 10.3390/ijms17111912

Table 1.

Ultra-high performance liquid chromatography (HPLC) analysis of the main phenolic compounds in cell suspension cultures of P. multiflorum.

No. Phenolic Compounds Concentration (µg/g DM)
Control PGR JA SA
Hydroxybenzoic acid
1 Gallic acid 681.45 ± 2.0 d 765.20 ± 1.5 c 819.12 ± 2.0 a 802.00 ± 1.5 b
2 Protocatechuic acid 302.15 ± 2.5 d 351.00 ± 1.0 c 425.41 ± 2.2 a 414.15 ± 1.0 b
3 β-Resorcylic acid 172.00 ± 1.6 d 235.50 ± 1.2 c 354.43 ± 1.5 a 340.15 ± 1.0 b
4 Syringic acid 101.75 ± 1.0 d 145.25 ± 1.5 c 280.22 ± 1.8 a 255.00 ± 2.0 b
5 Gentisic acid 185.15 ± 1.5 d 221.85 ± 1.0 c 325.00 ± 2.0 a 301.50 ± 1.5 b
Total 1442.50 d 1718.80 c 2204.18 a 2112.80 b
Hydroxycinnamic acid
6 Caffeic acid 165.25 ± 2.0 d 211.10 ± 1.0 c 265.55 ± 2.0 b 285.40 ± 1.0 a
7 p-Coumaric acid 95.00 ± 1.0 d 120.00 ± 1.5 c 205.10 ± 1.5 b 217.32 ± 1.2 a
8 Ferulic acid 312.45 ± 2.5 d 345.15 ± 2.0 c 415.50 ± 2.5 b 441.20 ± 1.4 a
9 Chlorogenic acid 72.05 ± 1.0 d 105.50 ± 1.1 c 211.12 ± 1.0 b 215.35 ± 1.0 a
10 m-Coumaric acid 40.00 ± 1.2 c 83.16 ± 1.0 b 106.00 ± 1.0 a 105.10 ± 1.5 a
11 o-Coumaric acid 102.20 ± 1.5 c 145.00 ± 1.2 b 202.10 ± 1.0 a 202.00 ± 1.0 a
Total 786.95 d 1009.91 c 1405.36 b 1466.37 a
Flavonols
12 Myricetin 245.25 ± 2.0 c 271.62 ± 1.5 b 385.50 ± 1.2 a 384.10 ± 1.5 a
13 Quercetin 312.10 ± 1.5 d 395.30 ± 2.1 c 455.55 ± 1.0 a 431.15 ± 2.0 b
14 Kaempferol 105.50 ± 1.0 d 152.10 ± 1.0 c 215.40 ± 2.0 a 201.55 ± 1.0 b
15 Naringenin 82.00 ± 1.0 d 95.50 ± 1.5 c 145.15 ± 1.0 a 120.55 ± 1.5 b
16 Rutin 525.50 ± 2.0 d 592.15 ± 2.5 c 675.00 ± 2.0 a 621.10 ± 2.5 b
17 Biochanin A 112.15 ± 1.5 c 125.00 ± 1.0 b 162.50 ± 2.0 a 160.00 ± 1.0 a
18 Formononetin 121.00 ± 1.8 c 145.20 ± 1.2 b 180.15 ± 1.0 a 180.51 ± 1.5 a
19 Hesperidin 132.55 ± 2.2 d 165.00 ± 1.5 c 195.50 ± 1.2 a 187.00 ± 1.0 b
Total 1636.05 d 1941.87 c 2414.75 a 2285.96 b
Other Phenolic compounds
20 Pyrogallol 925.00 ± 3.0 d 1012.44 ± 2.5 c 1121.15 ± 3.2 a 1101.00 ± 2.0 b
21 Resveratrol 212.00 ± 1.5 d 305.22 ± 2.0 c 414.50 ± 1.0 a 400.25 ± 1.5 b
22 Veratric acid 135.25 ± 1.0 d 194.10 ± 1.0 c 245.21 ± 1.5 a 212.15 ± 1.0 b
23 Vanillin 145.55 ± 1.0 b 146.00 ± 1.0 b 172.15 ± 1.2 a 171.00 ± 2.0 a
Total 1417.80 d 1657.76 c 1953.01 a 1884.40 b

Mean ± standard deviations within a row (a–d) followed by the same letters (alphabets) are not significantly different according to DMRT at p ≤ 0.05.