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Effect of TSN on GBM cell apoptosis. (A) Representative images of GBM cell apoptosis after TSN treatment. GBM cells were treated with either 10 nM TSN or an equal volume of vehicle for 48 h and evaluated for apoptosis by Annexin V/PI double staining; (B) Statistical analysis of the percentage of apoptotic GBM cells (* p < 0.05); (C,D) Western blot analysis of apoptosis-related proteins and estrogen receptor (ER) proteins in TSN-treated U87 and C6 cells.
